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human embryonic lung fibroblasts (hfl1) cell  (Galectin Therapeutics)

 
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    Galectin Therapeutics human embryonic lung fibroblasts (hfl1) cell
    Galectin-1 overexpression in <t>HFL1</t> cells. (a) Fluorescence images presented that galectin-1 was successfully overexpression in HFL1 cells (magnification: ×100). (b) qRT-PCR was conducted to measure galectin-1 mRnas expression. (c) Western blotting was conducted to measure galectin-1, TGF-β1 proteins. (d) The qualification of galectin-1, TGF-β1 proteins expression in ctrl and galectin-1-OE groups at different time. (e) Western blotting was conducted to measure α-SMA proteins expression in ctrl and galectin-1-OE groups at different time. The quantitative analysis of galectin-1 (f), TGF-β1 (g), and α-SMA (h) proteins. * p < .05, ** p < .01, *** p < .001.
    Human Embryonic Lung Fibroblasts (Hfl1) Cell, supplied by Galectin Therapeutics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+embryonic+lung+fibroblasts+(hfl1)+cell/human+embryonic+lung+fibroblasts++hfl1++cell/pmc10986763-15-5-0
    Average 90 stars, based on 1 article reviews
    human embryonic lung fibroblasts (hfl1) cell - by Bioz Stars, 2026-11
    90/100 stars

    Images

    1) Product Images from "Galectin-1 overexpression induces normal fibroblasts translate into cancer-associated fibroblasts and attenuates the sensitivity of anlotinib in lung cancer"

    Article Title: Galectin-1 overexpression induces normal fibroblasts translate into cancer-associated fibroblasts and attenuates the sensitivity of anlotinib in lung cancer

    Journal: Cell Adhesion & Migration

    doi: 10.1080/19336918.2024.2335881

    Galectin-1 overexpression in HFL1 cells. (a) Fluorescence images presented that galectin-1 was successfully overexpression in HFL1 cells (magnification: ×100). (b) qRT-PCR was conducted to measure galectin-1 mRnas expression. (c) Western blotting was conducted to measure galectin-1, TGF-β1 proteins. (d) The qualification of galectin-1, TGF-β1 proteins expression in ctrl and galectin-1-OE groups at different time. (e) Western blotting was conducted to measure α-SMA proteins expression in ctrl and galectin-1-OE groups at different time. The quantitative analysis of galectin-1 (f), TGF-β1 (g), and α-SMA (h) proteins. * p < .05, ** p < .01, *** p < .001.
    Figure Legend Snippet: Galectin-1 overexpression in HFL1 cells. (a) Fluorescence images presented that galectin-1 was successfully overexpression in HFL1 cells (magnification: ×100). (b) qRT-PCR was conducted to measure galectin-1 mRnas expression. (c) Western blotting was conducted to measure galectin-1, TGF-β1 proteins. (d) The qualification of galectin-1, TGF-β1 proteins expression in ctrl and galectin-1-OE groups at different time. (e) Western blotting was conducted to measure α-SMA proteins expression in ctrl and galectin-1-OE groups at different time. The quantitative analysis of galectin-1 (f), TGF-β1 (g), and α-SMA (h) proteins. * p < .05, ** p < .01, *** p < .001.

    Techniques Used: Over Expression, Fluorescence, Quantitative RT-PCR, Expressing, Western Blot

    Galectin-1 overexpression promotes tumor growth in vivo . (a) Imaging technology to monitor the growth of A549 ×enograft tumors. (b)The growth of xenograft tumors was determined by volume. (c) The growth of xenograft tumors was determined by tumor weight. vs A549 group, ** p < .01; vs A549+Anlotinib group, ## p < .01; vs gal-1 OE@HFL1:A549 group, && p < .01.
    Figure Legend Snippet: Galectin-1 overexpression promotes tumor growth in vivo . (a) Imaging technology to monitor the growth of A549 ×enograft tumors. (b)The growth of xenograft tumors was determined by volume. (c) The growth of xenograft tumors was determined by tumor weight. vs A549 group, ** p < .01; vs A549+Anlotinib group, ## p < .01; vs gal-1 OE@HFL1:A549 group, && p < .01.

    Techniques Used: Over Expression, In Vivo, Imaging

    IHC detected α-SMA, and Ki67 protein expression in vivo . (a) IHC staining of α-SMA protein in xenograft tumors. (b) The staining index of α-SMA. (c) IHC staining of Ki67 protein in xenograft tumors. (d) Positive cell percent of Ki67. Scale bar: 50 μm. (e) Western blotting was conducted to measure MMP2, MMP9, TGF-β1, VEGFA, PDGFA. (f) The qualification of proteins expression in different groups. vs A549 group, * p < .05, ** p < .01; vs A549+Anlotinib group, # p < .05, ## p < .01; vs gal-1 OE@HFL1:A549 group, & p < .05, && p < .01.
    Figure Legend Snippet: IHC detected α-SMA, and Ki67 protein expression in vivo . (a) IHC staining of α-SMA protein in xenograft tumors. (b) The staining index of α-SMA. (c) IHC staining of Ki67 protein in xenograft tumors. (d) Positive cell percent of Ki67. Scale bar: 50 μm. (e) Western blotting was conducted to measure MMP2, MMP9, TGF-β1, VEGFA, PDGFA. (f) The qualification of proteins expression in different groups. vs A549 group, * p < .05, ** p < .01; vs A549+Anlotinib group, # p < .05, ## p < .01; vs gal-1 OE@HFL1:A549 group, & p < .05, && p < .01.

    Techniques Used: Expressing, In Vivo, Immunohistochemistry, Staining, Western Blot

    Related Articles

    Over Expression:

    Article Title: Galectin-1 overexpression induces normal fibroblasts translate into cancer-associated fibroblasts and attenuates the sensitivity of anlotinib in lung cancer
    Article Snippet: Galectin-1 overexpression was conducted in Human embryonic lung fibroblasts (HFL1) cell.

    Fluorescence:

    Article Title: Galectin-1 overexpression induces normal fibroblasts translate into cancer-associated fibroblasts and attenuates the sensitivity of anlotinib in lung cancer
    Article Snippet: Galectin-1 overexpression was conducted in Human embryonic lung fibroblasts (HFL1) cell.

    Quantitative RT-PCR:

    Article Title: Galectin-1 overexpression induces normal fibroblasts translate into cancer-associated fibroblasts and attenuates the sensitivity of anlotinib in lung cancer
    Article Snippet: Galectin-1 overexpression was conducted in Human embryonic lung fibroblasts (HFL1) cell.

    Expressing:

    Article Title: Galectin-1 overexpression induces normal fibroblasts translate into cancer-associated fibroblasts and attenuates the sensitivity of anlotinib in lung cancer
    Article Snippet: Galectin-1 overexpression was conducted in Human embryonic lung fibroblasts (HFL1) cell.

    Western Blot:

    Article Title: Galectin-1 overexpression induces normal fibroblasts translate into cancer-associated fibroblasts and attenuates the sensitivity of anlotinib in lung cancer
    Article Snippet: Galectin-1 overexpression was conducted in Human embryonic lung fibroblasts (HFL1) cell.

    In Vivo:

    Article Title: Galectin-1 overexpression induces normal fibroblasts translate into cancer-associated fibroblasts and attenuates the sensitivity of anlotinib in lung cancer
    Article Snippet: Galectin-1 overexpression was conducted in Human embryonic lung fibroblasts (HFL1) cell.

    Imaging:

    Article Title: Galectin-1 overexpression induces normal fibroblasts translate into cancer-associated fibroblasts and attenuates the sensitivity of anlotinib in lung cancer
    Article Snippet: Galectin-1 overexpression was conducted in Human embryonic lung fibroblasts (HFL1) cell.

    Immunohistochemistry:

    Article Title: Galectin-1 overexpression induces normal fibroblasts translate into cancer-associated fibroblasts and attenuates the sensitivity of anlotinib in lung cancer
    Article Snippet: Galectin-1 overexpression was conducted in Human embryonic lung fibroblasts (HFL1) cell.

    Staining:

    Article Title: Galectin-1 overexpression induces normal fibroblasts translate into cancer-associated fibroblasts and attenuates the sensitivity of anlotinib in lung cancer
    Article Snippet: Galectin-1 overexpression was conducted in Human embryonic lung fibroblasts (HFL1) cell.



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    Galectin-1 overexpression in <t>HFL1</t> cells. (a) Fluorescence images presented that galectin-1 was successfully overexpression in HFL1 cells (magnification: ×100). (b) qRT-PCR was conducted to measure galectin-1 mRnas expression. (c) Western blotting was conducted to measure galectin-1, TGF-β1 proteins. (d) The qualification of galectin-1, TGF-β1 proteins expression in ctrl and galectin-1-OE groups at different time. (e) Western blotting was conducted to measure α-SMA proteins expression in ctrl and galectin-1-OE groups at different time. The quantitative analysis of galectin-1 (f), TGF-β1 (g), and α-SMA (h) proteins. * p < .05, ** p < .01, *** p < .001.
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    Image Search Results


    Galectin-1 overexpression in HFL1 cells. (a) Fluorescence images presented that galectin-1 was successfully overexpression in HFL1 cells (magnification: ×100). (b) qRT-PCR was conducted to measure galectin-1 mRnas expression. (c) Western blotting was conducted to measure galectin-1, TGF-β1 proteins. (d) The qualification of galectin-1, TGF-β1 proteins expression in ctrl and galectin-1-OE groups at different time. (e) Western blotting was conducted to measure α-SMA proteins expression in ctrl and galectin-1-OE groups at different time. The quantitative analysis of galectin-1 (f), TGF-β1 (g), and α-SMA (h) proteins. * p < .05, ** p < .01, *** p < .001.

    Journal: Cell Adhesion & Migration

    Article Title: Galectin-1 overexpression induces normal fibroblasts translate into cancer-associated fibroblasts and attenuates the sensitivity of anlotinib in lung cancer

    doi: 10.1080/19336918.2024.2335881

    Figure Lengend Snippet: Galectin-1 overexpression in HFL1 cells. (a) Fluorescence images presented that galectin-1 was successfully overexpression in HFL1 cells (magnification: ×100). (b) qRT-PCR was conducted to measure galectin-1 mRnas expression. (c) Western blotting was conducted to measure galectin-1, TGF-β1 proteins. (d) The qualification of galectin-1, TGF-β1 proteins expression in ctrl and galectin-1-OE groups at different time. (e) Western blotting was conducted to measure α-SMA proteins expression in ctrl and galectin-1-OE groups at different time. The quantitative analysis of galectin-1 (f), TGF-β1 (g), and α-SMA (h) proteins. * p < .05, ** p < .01, *** p < .001.

    Article Snippet: Galectin-1 overexpression was conducted in Human embryonic lung fibroblasts (HFL1) cell.

    Techniques: Over Expression, Fluorescence, Quantitative RT-PCR, Expressing, Western Blot

    Galectin-1 overexpression promotes tumor growth in vivo . (a) Imaging technology to monitor the growth of A549 ×enograft tumors. (b)The growth of xenograft tumors was determined by volume. (c) The growth of xenograft tumors was determined by tumor weight. vs A549 group, ** p < .01; vs A549+Anlotinib group, ## p < .01; vs gal-1 OE@HFL1:A549 group, && p < .01.

    Journal: Cell Adhesion & Migration

    Article Title: Galectin-1 overexpression induces normal fibroblasts translate into cancer-associated fibroblasts and attenuates the sensitivity of anlotinib in lung cancer

    doi: 10.1080/19336918.2024.2335881

    Figure Lengend Snippet: Galectin-1 overexpression promotes tumor growth in vivo . (a) Imaging technology to monitor the growth of A549 ×enograft tumors. (b)The growth of xenograft tumors was determined by volume. (c) The growth of xenograft tumors was determined by tumor weight. vs A549 group, ** p < .01; vs A549+Anlotinib group, ## p < .01; vs gal-1 OE@HFL1:A549 group, && p < .01.

    Article Snippet: Galectin-1 overexpression was conducted in Human embryonic lung fibroblasts (HFL1) cell.

    Techniques: Over Expression, In Vivo, Imaging

    IHC detected α-SMA, and Ki67 protein expression in vivo . (a) IHC staining of α-SMA protein in xenograft tumors. (b) The staining index of α-SMA. (c) IHC staining of Ki67 protein in xenograft tumors. (d) Positive cell percent of Ki67. Scale bar: 50 μm. (e) Western blotting was conducted to measure MMP2, MMP9, TGF-β1, VEGFA, PDGFA. (f) The qualification of proteins expression in different groups. vs A549 group, * p < .05, ** p < .01; vs A549+Anlotinib group, # p < .05, ## p < .01; vs gal-1 OE@HFL1:A549 group, & p < .05, && p < .01.

    Journal: Cell Adhesion & Migration

    Article Title: Galectin-1 overexpression induces normal fibroblasts translate into cancer-associated fibroblasts and attenuates the sensitivity of anlotinib in lung cancer

    doi: 10.1080/19336918.2024.2335881

    Figure Lengend Snippet: IHC detected α-SMA, and Ki67 protein expression in vivo . (a) IHC staining of α-SMA protein in xenograft tumors. (b) The staining index of α-SMA. (c) IHC staining of Ki67 protein in xenograft tumors. (d) Positive cell percent of Ki67. Scale bar: 50 μm. (e) Western blotting was conducted to measure MMP2, MMP9, TGF-β1, VEGFA, PDGFA. (f) The qualification of proteins expression in different groups. vs A549 group, * p < .05, ** p < .01; vs A549+Anlotinib group, # p < .05, ## p < .01; vs gal-1 OE@HFL1:A549 group, & p < .05, && p < .01.

    Article Snippet: Galectin-1 overexpression was conducted in Human embryonic lung fibroblasts (HFL1) cell.

    Techniques: Expressing, In Vivo, Immunohistochemistry, Staining, Western Blot